ABSTRACT
Objective To investigate the effect of laser‐doppler flowmetry (LDF) in the evaluation of established canine spi‐nal cord injury(SCI) model .Methods Totally 15 beagle dogs were randomly divided into the group A ,B and C .Through the im‐proved numerical control SCI apparatus ,different impacts and compression time were adopted to establish the different acute SCI models .The group A was 1 .2 m/s+5 mm+5 mm+15 mns ,the group B was 1 .2 m/s+5 mm+5 mm+10 mns ,while the group C wastakenasthecontrolgroup.LDFwasusedtomeasurethespinalcordbloodflow(SCBF)chart,thepathologicchangewasevalu‐ated by measuring the spinal cord necrosis area .Results The SCBF chart in 3 groups measured by LDF showed that the decrease of SCBF in the group A was most obvious ,the injury degree in the group A was more severe than those in the group B and C ,the difference was statistically significant(P<0 .05) .The motor evoked potential displayed that the decrease degree of SCBF was close‐ly related with the prognosis and motion function .Conclusion LDF could effectively judge the degree of spinal injury and SCBF sit‐uation ,which has a good application prospect in animal experiments and clinical field .
ABSTRACT
BACKGROUND:Studies have shown that neural stem cel transplantation combined with exercise training can promote the recovery of hindlimb motor function from spinal cord injury in rats, but its mechanism of action has not been fuly elucidated. OBJECTIVE:To investigate the effects of early exercise training combined with neural stem cel transplantation on the recovery of hindlimb motor function in rats with spinal cord injury. METHODS:Sixty Sprague-Dawley rats with spinal cord injury were randomly divided into three groups: control group (n=20, given conventional treatment after injury), cel transplantation group (n=20, given neural stem cel transplantation after injury), experimental group, (n=20, given neural stem cel transplantation combined with early exercise training after injury). Recovery of the hindlimb motor function was assessed by Basso, Beattie and Bresnahan scale and inclined plane test before and at 1, 7, 14, 21 days after injury. Western blot assay was used to detect caspase-3 and myeloperoxidase expression. Hematoxylin-eosin staining was done at 21 days after injury to observe the structure changes of the injured spinal cord. RESULTS AND CONCLUSION:(1) Scores of Basso, Beattie and Bresnahan scale and inclined plane test were significantly better in the experimental group than the cel transplantation group folowed by the control group (P < 0.05). (2) In the control group, the expression of caspase-3 and myeloperoxidase was significantly increased at 14 days after injury. In the cel transplantation, the expression of caspase-3 and myeloperoxidase was significantly higher than the experimental group (P < 0.05). (3) Pathological inflammation was reduced most in the experimental group folowed by the cel transplantation group. In the experimental group, the structure of injured spinal cord was improved and became relatively clear and intact. These findings indicate that neural stem cell transplantation combined with early exercise training can effectively promote the recovery of hindlimb motor function from spinal cord injury in rats, by reducing the expression of caspase-3 and myeloperoxidase and alleviating secondary lesion of the spinal cord.
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Objective To investigate indications and surgical methods of laparoscopic cholecystectomy (LC) in the management of acute cholecystitis. Methods Clinical data of 78 cases of acute cholecystitis treated by LC were retrospectively analyzed. Stones were impacted in the neck of the gallbladder in 65 cases. An anterograde cholecystectomy was performed in 47 cases, a retrograde cholecystectomy in 19 cases, and a subtotal cholecystectomy, in 12 cases. Results The laparoscopic operation was successfully accomplished in 74 cases, whereas a conversion to open surgery was required in 4. An abdominal drainage was used in 61 cases and was removed 24~48 hours after the surgery. The mean length of postoperative hospital stay was 4.8 days. Postoperative biliary leakage occurred in 1 case because of an injury of the common hepatic duct by electrocauterization, which was cured by open surgery with T-tube drainage for 6 months. No other severe complications happened. Conclusions With the exception of a small number of cases (such as Mirrizi's syndrome type Ⅲ or Ⅳ, the gallbladder totally or partially embedded in the liver, or suspected carcinoma of gallbladder), anterograde, retrograde or subtotal laparoscopic cholecystectomy can be safely applied to these with acute cholecystitis within 48 hours after the onset.